In-Vitro Fertilzation of Egg with Sperm.
After the retrieval, eggs are placed in the incubator for 3 to 6 hours and then inseminated with washed motile sperms which have been collected and prepared after the egg collection or taken from a frozen semen sample (Backup Arrangement). The eggs and sperms are placed in the incubator which try to mimic natural environment as closely as possible. (At a body temperature I.e. 37 degree centigrade and 5-6% Carbon Dioxide+ 5% Oxygen + 89-90% Nitrogen) . This modern triple gas incubation of embryos decreases the chances of abortions.
If husband has low sperm count, then ICSI procedure will be performed.
Fertilisation is the process of a sperm penetrating the egg. The egg, being fertilised, is now called an embryo. These embryos are observed further to be certain that they are dividing (cleaving) normally.